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Chemical modification of RNA beyond RNA drugs

January 24, 2023

Sehr geehrte Kolleginnen und Kollegen, wir laden herzlich zu einer Vortragsveranstaltung am Dienstag, den 25.10.2022, um 18 Uhr c.t. per ZOOM oder in den großen Hörsaal der Pharmazeutischen Institute, Corrensstraße 48 in Münster ein.

Meeting ID: 679 1003 4607
Passcode: DPhG

RNA plays a central role in the cell because, on the one hand, RNA is significantly involved in the production of proteins and, on the other hand, RNA is a regulator of gene expression. Despite its cellular importance, attempts to use RNA as a therapeutic agent have long been ridiculed because RNA is chemically unstable. In this talk, I will describe how chemical modification of RNA allows access to the RNA drug class. For this purpose, we will look at some representatives of the already approved RNA substance classes outside the currently prominent messenger RNA vaccines. The focus will be on splicing inhibitors, aptamers and small-interfering RNAs. The detection and quantification of RNA modifications in RNA drugs but also native RNA is possible by modern mass spectrometry. Yet, the temporal dynamics is not accessible with regular mass spectrometry. Here, we present nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS) for efficient, monoisotopic stable isotope labeling in both RNA and DNA in standard cell culture. With NAIL-MS, we design pulse chase experiments and study the temporal placement of modified nucleosides inhuman tRNAPhe, 18S rRNA and mRNA. This technology allows studies on RNA modification kinetics and stress response in a previously unachieved depths and is used to study the impact ofdrugs on the human RNA modification landscape.

Ich würde mich freuen, Sie zusammen mit Ihren Mitarbeitern begrüßen zu können.

Mit freundlichen Grüßen
Anna Junker

RG Westfalen-Lippe
Prof. Dr. Stefanie Kaiser
Institution of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany

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